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NTID 44214023
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💬 Talk to Our Bidding ExpertTranscriptomics Study rns Seq in Jorhat - Complete Tender Details
Find eligibility criteria, important deadlines, bidding requirements, and download official documents for "Transcriptomics Study rns Seq" in Jorhat, India.
- Opening Date : Wed 18th March, 2026
- Closing Date : Wed 18th March, 2026
- ₹ 0.00
- NTID 44214023
Tender Description
Documents
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Document-1
721.3 kBOil_India_ExtraDoc...Oil_India_ExtraDocument-GCO0449P23.pdf
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Document-2
721.3 kBOil_India_ExtraDoc...Oil_India_ExtraDocument-GCO0449P23.pdf
Costs
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EMD
22939.00 -
Document Cost
1000.00 -
Estimated Cost
Refer Documents
BOQ Items Total Items : 2 | Total Qty : 66
| # | Item | Quantity | Units |
|---|---|---|---|
| 1 | For undertaking transcriptomics study in rice under stress along with bioinformatics analysis | ||
| 2 | 1. Sample details Number of biological samples: 22. Tissue samples will be provided by AAU, Jorhat Each biological sample will be sequenced in triplicate (biological replicates) (Total libraries = 66) Sample collection and transport will be done using appropriate procedure, in multiple batches if required by service provider (To be borne by the vendor). 2. RNA extraction and library preparation Total RNA isolation using a standardized, vendor-validated protocol matching to globally accepting norms. RNA quality requirements: RIN 7.0 OD 260/280 = 1.8 2.1 rRNA depletion based library preparation Sequencing library specifications: Platform: Illumina (or equivalent) Read type: Paired-end Read length: 2 50 bp Minimum data output: ~20 GB per sample Base quality: 97% bases Q30 3. Sequencing quality requirements 90% reads mapped to reference genome Duplication rate 20% Adapter contamination 1% 4. Bioinformatics analysis (end-to-end, publication ready) Primary analysis Raw data delivery: FASTQ files Quality control: FastQC + MultiQC Adapter trimming and quality filtering Alignment to reference genome (organism to be specified by institute) Output: Aligned BAM files Expression analysis Gene-level quantification (raw counts + normalized values: TPM/FPKM) Differential gene expression analysis: Group-wise contrasts (to be defined by institute) Log FC, p-value, adjusted p-value (FDR) Outputs: Differentially expressed gene lists (Up- and Down-regulated) CSV/Excel format tables Downstream analysis PCA, hierarchical clustering, correlation plots anything more you need. Volcano plots, MA plots, heatmaps Functional annotation: GO enrichment KEGG / pathway analysis Read coverage visualization files (BigWig) Final deliverables Complete analysis report: PDF + interactive HTML All scripts and pipeline details for reproducibility Note: Peak calling, motif discovery, and differential binding analysis are not applicable to standard RNA-sequencing and are not required under this scope. | 66 | nos |
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